| Issue | #541 |
| Published | June 2007 |
| Cover Price | 2.99 USD |
| Pages | 36 |
| Editing | Axel Alonso; Michael O'Connor (Assistant); Daniel Ketchum (Assistant) |
| Characters | Spider-Man |
| Genre | superhero |
| Pencils | Ron Garney |
| Inks | Ron Garney |
| Letters | Typeset |
| Notes | Cover sports Back in Black trade dress. |
| Genre | superhero |
| Pencils | Ron Garney |
| Inks | Ron Garney |
| Letters | Typeset |
| Characters | Spider-Man; GUESTS: Mary Jane Parker; Aunt May Parker; VILLAIN: Kingpin |
| Synopsis | Now that he knows the Kingpin ordered the hit that brought down Aunt May, Spidey takes the goon who shot sniper Jake Martino down to the sewers and mercilessly pumps him for information; Returning to the hospital and finding that Aunt May is not getting any better, with their money running out, Peter decides to take drastic measures; He gives Aunt May a transfusion of his own blood as this has helped her recover in the past; Knowing that Spidey is onto him, Kingpin pays off the prison guards to bring him his clothes and open the doors so he can escape; As he is walking out, Spider-Man arrives for a confrontation. |
| Genre | superhero |
| Script | J. Michael Straczynski |
| Pencils | Ron Garney |
| Inks | Bill Reinhold |
| Colors | Matt Milla |
| Letters | Virtual Calligraphy's Cory Petit |
| Characters | Spider-Man; Kingpin |
| Genre | superhero |
| Pencils | Ron Garney |
| Inks | Ron Garney |
| Letters | Typeset |
| Notes | Presents full page cover art for next issue. |